dextran hydrodynamic radius

dextran hydrodynamic radius

Pharmacol Pharm. 113, 81-86. By using this website, you agree to our This situation is mainly the result of using dextans, solvent, additives, etc. (2007). Fusco V, Quero GM, Cho GS, Kabisch J, Meske D, Neve H, Bockelmann W, Franz CM. Crini, G. (2005). Hydrodynamic Radius If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. 2.It can be found from the figure that at the start-up stage of the shaft, the hydrodynamic effect (HE) is weak, so the HP is not enough to support the shaft, which This conjecture found to be correct. 4K was 10.611.68 and 13.440.18M in human and pig skin, respectively. Bookshelf Ghimici, Luminita; Nichifor, Marieta; Wolf, Bernhard (2009). In the case of the work performed in references [34, 35, 36] they studied using the number average molecular weight, which rearranges the value of a and forces it to enter into unbranched or linear molecules, on the other hand, if molecular weights are used the value would be less than 0.5. The lower molecular weight of FITC-dextran permeated more on both human and pig skin. Federal government websites often end in .gov or .mil. WebThomas Gutberlet works at Jlich Centre for Neutron Science (JCNS-2), Forsch and is well known for Lipoteichoic Acid, Neutron Reflectometry and Neutron Reflectivity. Synthesis of dextran by dextransucrase treated, sucrose-supplemented skimmed milk revealed the promising potential of dextran as a food additive. 2017 Dec 12;27(1):73-78. doi: 10.1007/s10068-017-0274-0. ; van Nostrum, C.F. Search The proportionality between []0 and M0.5 is obtained as a limiting law for the no draining regime. Prausnitz MR, Langer R. Transdermal drug delivery. Micropores are produced by the arrangement of microelectrodes on the skin at precise dimensions, then RF energy generated by alternating currents induces ionic vibrations between electrodes with positive or negative charges. 4h after microporation, the sizes of the micropore decreased slightly. This result could reflect the presence of some aggregated species within the lysozyme sample solution. The mechanism of the dextran-induced red blood cell Aggregation. The Dextrans intrinsic viscosity is determined as a function of the molecular weight in water and NaOH 0.5 M see reference [1]. All authors read and approved the final manuscript. Whiteside PJD, Chininis JA, Schellenberg MW, Qian C, Hunt HK. ; Ross-Murphy, S.B. European Polymer Journal, 43, 1744-1753. WebFITC Products. (2008). 43-1, 115-147. principles of dynamic light scattering Pig skins (Micropig Franz cell membrane) were supplied by Medi Kinetics Co., Ltd. (Pyeongtaek, Korea). Materiaux 1-5. 2023 Feb 17;12(4):861. doi: 10.3390/foods12040861. Typical examples are the classical size-independent combinations like the Flory parameter, which that combine the intrinsic viscosity, [], and the gyration radius, Rg: These quantities have been proposed along the years, by different eminent scientists, after whom they are named. Mainaa, Ndegwa Henry; Pitknen, Leena; Heikkinen, Sami; Tuomainen; Pivi; Virkki, Liisa; Tenkanen, Maija (2014). Leiva, Angel; Muoz, Natalia; Urza, Marcela; Gargallo, Ligia; Radic, Deodato (2010). Based on these results a difference of approximately 1015% between the amounts of drugs permeated through the human skin and pig skin models should be considered during human clinical trials. Viscosity of in water solution polysaccharides depends on intrinsic characteristics of the biopolymer (such as molecular weight, volume, size, shape, surface charge, deformation facility, esterification degree, and galacturonic content) and on ambient factors (such as pH, temperature, ionic strength, solvent, etc.). 2011;16(2):518. Just in case anyone wants to use a different molecular weight to estimate the hydrodynamic size: the calculator is integrated into the classic Zeta Drug Deliv Syst. Bose, A.; Rollings, J. E.; Caruthers, J. M.; Okos, M. R.; Tsao, T. G. (1982). Characterization of high molecular weight dextran produced by Weissella cibaria CMGDEX3. Doke SK, Dhawale SC. For rigid macromolecules, it is also conventional to combine a solution property with the volume of the particle itself, or with a quantity directly derived from it [54, 55, 56]. Aqueous solutions of amphiphilic polysaccharides: Concentration and temperature effect on viscosity. Dextran Influence of molecular weight on The equation 4 can be rearranged and resulted in a modified M-H equation as follows: where the value qM-H is the polydispersity correction factor, and it is a statistical function of the molecular weight distribution. Ortega, A.; Garca de la Torre, J. Progr. 2023 BioMed Central Ltd unless otherwise stated. 2013 May;64(3):346-54. doi: 10.3109/09637486.2012.734289. Typically, the hydrodynamic diameter of the micelles is about 100 nm and the critical micelle concentration is about 10 mg/L. In this work, we evaluate the maximum radius of macromolecules able to enter into HeLa cells following a plasma treatment using a size exclusion approach with dextran molecules. "Dextrans in Aqueous Solution. 56 The linkage that the enzyme dextranase breaks down is indicated by the black arrow. Physicochemical studies of oligodextran. Experimental Review on Intrinsic Viscosity Measurements and Temperature Effect.". The value of Mv is different from Mn, Mw, and Mz in a polydisperse polymer sample [38, 60, 61, 62]. Dextrans and their derivatives find an interest in clinical applications, as well as excipients in tablets in the pharmaceutical industry [5]. (1999). WebIn this regard, we chose as crowding agents a number of polymers with hydrodynamic dimensions comparable to those of iRFP713 in different structural states: polyethylene glycol8,000(PEG-8000)withaneffectivehydrodynamicradiusof24.5 1.9(Devanand & Selser, 1991), Dextran-40 and Dextran-70 with hydrodynamic radius of 44.550 and 5864 , Although, in literature there is much information on hydrodynamic measurements from determinations viscosity; very few of them evaluate the situation at different temperatures. The intrinsic viscosity can be easily calculated by the Solomon-Ciuta single-point equation [44, 45]. Nanoparticles in magnetic resonance imaging: from simple to dual Polymer molecular weight distribution. Coviello, T.; Matricardi, P.; Marianecci, C.; Alhaique, F. (2007). Cite this article. Actually, everything depends on solvent you want to use and temperature. If you want to know gyration (Rg)or hydrodynamic (Rh) radius, you should ; Ghosh, T.K. Recent Pat. Drug Deliv. Table 3 shows the intrinsic viscosity for the rest of the dextrans obtained by using the single-point method, measured at different temperatures. Thus, it is a common practice to express the frictional coefficient of rigid structures as. Pure Appl. Transdermal delivery of FITC-Dextrans with different molecular weights using radiofrequency microporation, https://doi.org/10.1186/s40824-020-00201-7, http://creativecommons.org/licenses/by/4.0/, http://creativecommons.org/publicdomain/zero/1.0/. Intrinsic viscosity determination offers a simple and useful method that requires low cost equipment and yields useful information on soluble macromolecules. Enhanced topical delivery of small hydrophilic or lipophilic active agents and epidermal growth factor by fractional radiofrequency microporation. The Mark-Houwink a constant is close to 0.5 in poor solvents. The Hydrodynamic Radii of Macromolecules and Their Effect on Red Blood Cell Aggregation. 2010;4(1):117. In addition, microporated pig skin permeated larger amounts of FITC-dextran than human skin, this can be attributed to the differences in structure between the human and pig skin; the pig skin layer is thinner (SC, 813m) than the human skin (SC, 1017m) and the pig hair follicle is longer (3871m) than the human hair follicle (18m). Table1 shows TEWL values measured immediately after the microporation of human and pig skins. The Mark-Houwink parameters is calculated molecular weights range from 8.8 to 200kDa. J. On the molecular weight distribution of dextran T-500. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. Sintov AC, Krymberk I, Daniel D, Hannan T, Sohn Z, Levin G. Radiofrequency-driven skin microchanneling as a new way for electrically assisted transdermal delivery of hydrophilic drugs. Ioan, C.E. Principles of Size Exclusion Chromatography SEC (GFC (1999). Ionic Polymers Based on Dextran: Hydrodynamic Properties in Aqueous Solution and Solvent Mixtures. The biological activity of cationic liposomes in drug delivery and toxicity test in animal models. Soc., 21, 1, 78-86. The calculated dextran molecular weights are corroborated by those tabulated data by the manufacturer with a relative error lower than 10%, being the exception dextrans 580 and 2000kDa with the highest relative error. Hydrodynamic properties, such as the intrinsic viscosity, [], and equilibrium solution properties such as the hydrodynamic radius RH can be combined to construct dimensionless quantities that are universal since they are of being independent of the macromolecular particle size, while they depend more or less sensitively on its shape [51]. Before Advances in Carbohydrate Analysis, 1, 63-144. 2005;22(4):5505. Surface morphology study of dextran by scanning electron microscopy showed the porous web like structure. Bahary, W.S. dextran hydrodynamic radius. dextran US20230040324A1 AMINOACRIDINE AND AMINOPYRENE DYES The fast increasing of these polyglucosans for medical, industrial and research purposes motivated a survey of the types obtainable. 8580 Macromolecules 34, Notes - NIST H&E stained images were analyzed using Image J software. In order to understand the behavior of this biopolymer in water and to support changes at the M-H parameters with temperature, the hydrodynamic parameters are analyzed (hydrodynamic radius, (RH); Simha number, ((a/b)); Perrin parameter (P); Flory parameter (0); and stiffness chain (dln[]/dT)). Normally, the skin loses very little water loss whereas damaged skin loses more [22]. Solutions of dextrans and blue dextrans (0.21.0 g/L) were prepared in 0.200 M NaCl (aq). Int J Food Sci Nutr. Dextran, Tetramethylrhodamine, 2,000,000 MW, Lysine (1991). Palmuti Braga Vettoria, Mary Helen; Martins Franchetti, Sandra Mara; Contiero, Jonas (2012). (1954). The a value is higher than calculated in this work, because these calculations were performed with number average molecular weight (Mn) which rearranges the value of a and forces him to enter into unbranched or linear molecules (a > 0.5), on the other hand, if molecular weights (Mw) ware used to value would be less than 0.5. Journal of Chemical Education, 80(9), 1036-1038. Transdermal delivery is of great importance for the effective delivery of bioactive or therapeutic agents into a body. Porcine wound models for skin substitution and burn treatment. For a given polymer-solvent pair, the theta condition is satisfied at a certain temperature, called the theta () temperature or theta point. Epub 2012 May 28. WebThe study of biopolymers as dextran in aqueous solution, is effectively determined by intrinsic viscosity [] measurements at different temperatures. The cumulative permeation of FITC-dextran 10K was 4.820.32M, in the human skin and 5.920.46M in the pig skin. Privacy Furthermore, the influence of temperature on the intrinsic viscosity is given by the chain flexibility parameter (dln[]/dT), which gives information about the conformation of the macromolecule chain in solution [62]. Amersham Biosciences, AA Edition, www.amershambiosciences.com. These results indicate that healing and regeneration occurred in the human cadaver skin after the microporation. Harding, Stephen E. (2005). Polysaccharides-based nanoparticles as drug delivery systems. Thus, it is accepted that, for every flexible-chain polymer in a (ideal) solvent, there is a universal value of 0 = 2.1-2.5x1023mol-1 [52]. 2012;27(3):20212. Webdextran fraction and the final product depend on changes in the hydrodynamic volume of the molecules after substitution. (more or less, with a hydrodynamic radius of less than 100 nm) to cross from the vasculature into the interstitium. Epub 2022 Feb 2. Biomacromolecules 8, 2464-2475. 2019;26(1):25261. Formation of dextran hydrogels by crystallization. Int J Pharm Investig. Chem. Sung-Wook Choi. Appl Biochem Biotechnol. The correct way to interpret what Mark-Houwink parameters is determined graphically ln [] versus ln Mw, where Mw is the molecular weight provided by manufacturer, and calculate the percentage relative error (%RE) respect to Mw. Dextran 670 GPC Standard | Pharmacosmos Dextran Dextran; Weissella cibaria; exopolysaccharide; prebiotic; skimmed milk. This device can generate microchannels from the SC through to the outer dermis and allow transdermal delivery of active agents. Takara, Andres; Acosta, Adolfo; Masuelli, Martin A. The dextran has the advantage in terms of low cytotoxicity, excellent biocompatibility, and water-solubility. (2003). Molecular weight (M v) and hydrodynamic properties can be calculated from there. Colloid Polym. Determination of the intrinsic viscosity has been the most used measurement in the last 60 years. Figure2 shows TEWL values measured before and immediately following the treatment of human cadaver skin with the microporation device. What is hydrodynamic radius (1954). Kuge Takashi; Kimie Kobayashi; Shinichi Kitamura; Hiroshi Tanahashi.

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